产品货号：
GS2218
中文名称：
EcoICRI内切酶
英文名称：
EcoICRI
产品规格：
200U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：EcoICRI内切酶，EcoICRI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | GAG↑CTC |
| 来源 | Escherichia coli ICR |
| Assayed on | Lambda DNA(HindIII-digest) |
| Optimal buffer | G(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 37℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol;Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a.of enzyme for 16hoursat 37℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | Ecl136II,Eco53kI,Psp124BI?^,SacI^,SstI^ |
相关搜索：EcoICRI内切酶，EcoICRI限制性内切酶